Consensus sequence

Sometimes there is the need to create a consensus sequence for an individual where the sequence incorporates variants typed for this individual. This is possible using the consensus command.

We need the reference sequence reference.fa in the fasta format and an indexed VCF with the variants calls.bcf. The command is:

cat reference.fa | bcftools consensus calls.vcf.gz > consensus.fa

This assumes we have already made the calls, normalized indels and filtered. There is another page which goes deeper and is devoted just to this, but in brief, the variant calling command in its simplest form is:

# call variants
bcftools mpileup -Ou -f reference.fa alignments.bam | bcftools call -mv -Oz -o calls.vcf.gz
bcftools index calls.vcf.gz

# normalize indels
bcftools norm -f reference.fa calls.vcf.gz -Ob -o calls.norm.bcf

# filter adjacent indels within 5bp
bcftools filter --IndelGap 5 calls.norm.bcf -Ob -o calls.norm.flt-indels.bcf

Feedback

We welcome your feedback, please help us improve this page by either opening an issue on github or editing it directly and sending a pull request.